Electrophoretic isolation of β-casein and optimization of a radial immunodiffusion test for bovine milk quality control

Beta-casein (β-CN) is a major dairy protein subject to preferential degradation during storage. Our study aimed isolate β-casein from bovine milk under dissociative conditions by the electrophoretic technique using Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) and optimize an immunochemical such as Mancini radial immunodiffusion in order monitor evolution of this For purpose, series physicochemical bacteriological analyses revealed conformity with international standards. This allowed us use quality for more detailed studies proteins, particular β-casein, which was selected marker quality. The total casein mixed samples isolated then submitted separation. Various combinations acrylamide bisacrylamide were used one corresponding ratio 8 g / 230 mg retained. A deposit 833 µg provided adequate b-casein concentration (300 µg) consistent animal immunization. Thirty Wistar rats subjected immunization protocol 35 days. obtained antisera assay our at different storage times ranging 3 h 69 h/+4°C. decrease content 0.85 ml-1 0.32 detected. These results demonstrate vulnerability low temperatures provide information on origin its application or cheese production. In addition, confirms usefulness techniques test determination β-casein.